DATASHEET
Host:
Mouse
Target Protein:
KRT19
Clonality:
Monoclonal
Isotype:
IgG1
Entrez Gene:
3880
Swiss Prot:
P08727
Source:
This antibody is generated from a mouse immunized with a recombinant protein of human.
Purification:
Purified by Protein G.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
VALIDATION IMAGES
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeled with KRT19 (979CT14.3.1) Monoclonal Antibody (bsm-51308M) at 1/25 dilution, followed by secondary antibody (green) and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with KRT19 (979CT14.3.1) Monoclonal Antibody (bsm-51308M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.