bsm-51281M [Primary Antibody]
CDKN1B (10B7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: CDKN1B

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 1027

Swiss Prot: P46527

Source: This CDKN1B antibody is generated from a mouse immunized with CDKN1B recombinant protein.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 22


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Huihui Wang. et al. Regulation of GDF9 and CDKN1B expression in Tibetan sheep testes during different stages of maturity. Gene Expr Patterns. 2021 Nov;:119218Read more>>
  • Ruixue Zhang. et al. The miR-15b-5p/miR-379-3p-FOXO axis regulates cell cycle and apoptosis in scleral remodeling during experimental myopia. J TRANSL MED. 2024; 22: 71Read more>>
VALIDATION IMAGES

Lane 1: MCF-7 Cell lysates; Lane 2: U266 Cell lysates; probed with CDKN1B (10B7) Monoclonal Antibody, unconjugated (bsm-51281M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed, paraffin embedded human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDKN1B (10B7) Monoclonal Antibody (bsm-51281M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.