DATASHEET
Host:
Mouse
Target Protein:
ACE2
Immunogen Range:
631-805/805
Clonality:
Monoclonal
Isotype:
IgM
Entrez Gene:
59272
Swiss Prot:
Q9BYF1
Source:
Purified His-tagged ACE2 protein was used to produced this monoclonal antibody.
Purification:
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. (Microbial infection) Acts as a receptor for SARS coronavirus/SARS-CoV and human coronavirus NL63/HCoV-NL63.
PRODUCT SPECIFIC PUBLICATIONS
- Giovanni Sarnelli. et al. Intranasal administration of Escherichia coli Nissle expressing the spike protein of SARS-CoV-2 induces long-term immunization and prevents spike protein-mediated lung injury in mice. BIOMED PHARMACOTHER. 2024 May;174:116441Read more>>
VALIDATION IMAGES
Lane 1: 293 Cell lysates; Lane 2: K562 Cell lysates; Lane 3: MCF-7 Cell lysates; Lane 4: SK-BR-3 Cell lysates; Lane 5: T47D Cell lysates; Lane 6: MDA-MB-453 Cell lysates; Lane 7: mouse testis lysates; probed with ACE2 (881CT16.4.4) Monoclonal Antibody, unconjugated (bsm-51221M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Recombinant human ACE2 protein, His (HEK293) probed with ACE2 Monoclonal Antibody, Unconjugated (bsm-51221M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.