bsm-51061M [Primary Antibody]
ATG4D (2C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: ATG4D

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 84971

Swiss Prot: Q86TL0

Source: This ATG4D monoclonal antibody is generated from mouse immunized with ATG4D recombinant protein.

Purification: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. Cysteine protease ATG4D, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis. The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, ROS, mitophagy and cell viability.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 53


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: A431; Lane 2: HL-60 cell lysates; probed with ATG4D (222CT15.4.1) Monoclonal Antibody, unconjugated (bsm-51061M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.