bsm-51003M [Primary Antibody]
Myc Tag (9E10) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Myc Tag

Clonality: Monoclonal

Isotype: IgG1

Source: KLH conjugated synthetic peptide encoding c-Myc tag was used as antigen.

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. The anti-Myc Tag monoclonal antibody (Clone 9E10) provides a simple solution to detect the expression of a Myc-tagged protein in cells.

Size: 100ul

Concentration: 0.5ug/ul

Cross Reactive Species: Others

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

LMyc-Tagged Fusion Protein Overexpression E.coli Lysates probed with Myc Tag Monoclonal Antibody, Unconjugated (bsm-51003M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


4 ul Tags Positive Control Whole Cell Lysate (bs-41230P) per lane probed with Myc Tag monoclonal antibody respectively, unconjugated (bsm-51003M) at 1:10000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.