VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
RSC96 probed with alpha Tubulin (4G1) Monoclonal Antibody, Unconjugated (bsm-50179M) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human cervical carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Lane 1: Hela; Lane 2: 293T; Lane 3: MDA-MB-231; Lane 4: A549; Lane 5: A431; Lane 6: RSC96; Lane 7: VSC4.1; Lane 8: ASPC-1; Lane 9: B16; Lane 10: BV2; Lane 11: H9C2; Lane 12: Hep2G;Lane 13: K562; Lane 14: U937; probed with alpha Tubulin (4G1) Monoclonal Antibody, unconjugated (bsm-50179M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Lane 1 : HeLa Cell Lysate; Lane 2 : 293T Cell Lysate; Lane 3 : C6 Cell Lysate; Lane 4 : NIH3T3 Cell Lysate probed with alpha Tubulin (4G1) Monoclonal Antibody, unconjugated (bsm-50179M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at room temperature.