Host: Mouse
Target Protein: Alpha-synuclein
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 6622
Swiss Prot: P37840
Source: Recombinant human Alpha-synuclein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
Predicted Molecular Weight: 14
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Rat Hippocampus tissue lysates probed with Alpha-synuclein Monoclonal Antibody, Unconjugated (bsm-42005M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Alpha-Synuclein Monoclonal Antibody, Unconjugated(bsm-42005M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Alpha-Synuclein Monoclonal Antibody, Unconjugated(bsm-42005M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Alpha-Synuclein Monoclonal Antibody, Unconjugated(bsm-42005M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Alpha-Synuclein Monoclonal Antibody, Unconjugated(bsm-42005M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with Alpha-synuclein monoclonal antibody, unconjugated (bsm-42005M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.