bsm-33425M

DATASHEET

Host: Mouse

Target Protein: CDKN1B

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1027

Swiss Prot: P46527

Source: Recombinant human CDKN1B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1; while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.

Size: 100ul

Concentration: 1ug/ul

Applications: IHC(1:100-500)

Predicted Molecular Weight: 22

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.