bsm-33400M [Primary Antibody]
Collagen I Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Collagen I

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1277

Swiss Prot: P02452

Source: KLH conjugated synthetic peptide derived from human Collagen I

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)
IF()

Predicted Molecular Weight: 139 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Si-lin Lv. et al. Lp-PLA2 inhibition prevents Ang II-induced cardiac inflammation and fibrosis by blocking macrophage NLRP3 inflammasome activation. Acta Pharmacol Sin. 2021 Jul;:1-17Read more>>
  • Ruidong Ge. et al. Quantitative proteomics reveals potential anti-inflammatory protein targets of radial extracorporeal shock wave therapy in TNF--induced model of acute inflammation in primary human tenocytes. HELIYON. 2022 Dec;8:e12008Read more>>
  • Chenglin Liang. et al. Spatiotemporal transformable nano-assembly for on-demand drug delivery to enhance anti-tumor immunotherapy. ASIAN J PHARM SCI. 2024 Feb;:100888Read more>>
  • Jingwen Hui. et al. 3D printed fibroblast-loaded hydrogel for scleral remodeling to prevent progression of myopia. J MATER CHEM B. 2024 FebRead more>>
  • etl Seher Uysal. et al. Investigation of healing strategies in a rat corneal opacity model with polychromatic light and stem cells injection. J PHOTOCH PHOTOBIO B. 2024 Apr;253:112874Read more>>
  • Jie Huang. et al. DP7-C/mir-26a system promotes bone regeneration by remodeling the osteogenic immune microenvironment. ORAL DIS. 2024 MaRead more>>
  • Mei Zhang. et al. Protective Effects of Resveratrol on Cytotoxicity of Mouse Hepatic Stellate Cells Induced by PM2.5. ATMOSPHERE-BASEL. 2024 May;15(5):588Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin-embedded Rat Skeletal Muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (bsm-33400M) at 1:400 overnight at 4°C, followed by a secondary antibody, Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.


Paraformaldehyde-fixed, paraffin-embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (bsm-33400M) at 1:400 overnight at 4°C, followed by a secondary antibody, Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.