Host: Mouse
Target Protein: ATG5/APG5L
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 9474
Swiss Prot: Q9H1Y0
Source: Recombinant human ATG5 Protein
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications: IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 32
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5/APG5L) Polyclonal Antibody, Unconjugated (bsm-33386M) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-mouse IgG antibody (bs-0296G-AF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5/APG5L) Polyclonal Antibody, Unconjugated (bsm-33386M) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-mouse IgG antibody (bs-0296G-AF488) for 90 minutes, and DAPI for nuclei staining.