bsm-33337M
[Primary Antibody]
ERK1/2 Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: ERK1/2
Immunogen Range: 151-250/380
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 5595
Swiss Prot: P27361
Source: KLH conjugated synthetic peptide derived from human ERK1/2
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(WB=1:500-1000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Kai Li. et al. Novel Multitarget Directed Tacrine Hybrids as Anti-Alzheimers Compounds Improved Synaptic Plasticity and Cognitive Impairment in APP/PS1 Transgenic Mice. Acs Chem Neurosci. 2020;11(24):4316C4328Read more>>
- Xuejiao Zhou. et al. The novel ALK inhibitor ZX\29 induces apoptosis through inhibiting ALK and inducing ROS\mediated endoplasmic reticulum stress in Karpas299 cells. 2020 Nov 02Read more>>
- Fengyan Huang. et al. SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas. J Cancer. 2021; 12(18): 5439C5453Read more>>
- Yanjie Wang. et al. Bisdemethoxycurcumin attenuates OVA_induced food allergy by inhibiting the MAPK and NF__B signaling pathways. EXP THER MED. 2022 Jun;23(6):1-8Read more>>
- Huanshan He. et al. Lactoferrin alleviates spermatogenesis dysfunction caused by bisphenol A and cadmium via ameliorating disordered autophagy, apoptosis and oxidative stress. INT J BIOL MACROMOL. 2022 Dec;222:1048Read more>>
- Qianfeng Liu. et al. Perfluoroalkyl substances promote breast cancer progression via ER and GPER mediated PI3K/Akt and MAPK/Erk signaling pathways. ECOTOX ENVIRON SAFE. 2023 Jun;258:11498Read more>>
- Xinyun Qin. et al. Regulation of the intestinal flora using polysaccharides from Callicarpa nudiflora Hook to alleviate ulcerative colitis and the molecular mechanisms involved. INT J BIOL MACROMOL. 2024 Feb;258:128887Read more>>
- Cheng Wenchao. et al. The Impact of Dipyridamole on Disease-Associated Microglia Phenotypic Transformation in White Matter Lesions Induced by Chronic Cerebral Hypoperfusion. NEUROCHEM RES. 2023 Dec;:1-14Read more>>
- Chenhao Xu. et al. Human umbilical cord mesenchymal stem cells alleviate fatty liver ischemia-reperfusion injury by activating autophagy through upregulation of IFN. CELL BIOCHEM FUNCT. 2024 Jun;42(4):e404Read more>>
- Yin-Ku Lin. et al. Systematic establishment of the relationship between skin absorption and toxicity of furanoids via in silico, in vitro, and in vivo assessments. ENVIRON RES. 2024 Nov;261:119757Read more>>
- Qinbing Xue. et al. Lycorine (Lycoris radiata)a unique natural medicine on breast cancer. J CELL MOL MED. 2024 Aug;28(16):e70032Read more>>
VALIDATION IMAGES
Lane 1: NIH/3T3 cell lysates probed with ERK1/2 Monoclonal Antibody, Unconjugated (bsm-33337M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: A431 Cell cell lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Cerebrum lysates; Lane 4: SiHa cell lysates ; Lane 5: MCF-7 cell lysates;Lane 6: HUVEC cell lysates probed with ERK1/2 Polyclonal Antibody, Unconjugated (bsm-33337M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2 Monoclonal Antibody, Unconjugated (bsm-33337M) at 1:2000 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2 Monoclonal Antibody, Unconjugated (bsm-33337M) at 1:2000 overnight at 4°C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with ERK1/2 monoclonal antibody, unconjugated (bsm-33337M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with ERK1/2 monoclonal antibody, unconjugated (bsm-33337M) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.