Host: Mouse
Target Protein: Gamma Tubulin
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 7283
Swiss Prot: P23258
Source: KLH conjugated synthetic peptide derived from human Gamma Tubulin
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end.
The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 50 kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: A549 cell lysates;Lane 2: MCF-7 cell lysates; Lane 3: U87MG cell lysates probed with Gamma Tubulin Polyclonal Antibody, Unconjugated (bsm-33313M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin-embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Gamma Tubulin Monoclonal Antibody, Unconjugated (bsm-33313M) at 1:2000 overnight at 4°C, followed by DAB staining.
Paraformaldehyde-fixed, paraffin-embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma Tubulin) Monoclonal Antibody, Unconjugated (bsm-33313M) at 1:2000 overnight at 4°C, followed by DAB staining.
Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Cerebellum tissue lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human A549 cell lysates probed with Gamma Tubulin Monoclonal Antibody, Unconjugated (bsm-33313M) at 1:3000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.