Host: Mouse
Target Protein: Actin, alpha skeletal muscle
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 58
Swiss Prot: P68133
Source: KLH conjugated synthetic peptide derived from human ACTA1
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1mg/ml
Applications:
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()
ICC(1:100-500)
Predicted Molecular Weight: 42
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Cow
Sheep
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated (bsm-33297M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated (bsm-33297M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.