bsm-33281M-BF488 [Conjugated Primary Antibody]
AKT (Ser473)(9H11) Monoclonal Antibody, AbBy Fluor® 488 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: AKT (Ser473)

Modification Site: Ser473

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 207

Swiss Prot: O57513

Source: KLH conjugated Synthesised phosphopeptide derived from human AKT around the phosphorylation site of Ser473

Purification: Purified by Protein G.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Conjugation: AbBy Fluor® 488

Excitation/ Emission: 499nm/519nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 56 kDa


Cross Reactive Species: Human
Mouse
Rat
Others

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang H et al. Ginsenoside Rb1 promotes the growth of mink hair follicle via PI3K/AKT/GSK-3β signalingpathway. Life Sci. 2019 Jul 15;229:210-218. Read more>>
  • Sun Y et al. Shenmai Injection Supresses Glycolysis and Enhances Cisplatin Cytotoxicity in Cisplatin-Resistant A549/DDP Cells via the AKT-mTOR-c-Myc Signaling Pathway. Biomed Res Int. 2020 Jun 20;2020:9243681.Read more>>
  • Le Tian. et al. Luteolin as an adjuvant effectively enhances CTL anti-tumor response in B16F10 mouse model. Int Immunopharmacol. 2021 May;94:107441Read more>>
  • Qun Lin. et al. Silencing CTNND1 Mediates Triple-Negative Breast Cancer Bone Metastasis via Upregulating CXCR4/CXCL12 Axis and Neutrophils Infiltration in Bone. Cancers. 2021 Jan;13(22):5703Read more>>
  • Chen, Yao. et al. The potential effects and mechanisms of_hispidulin in the treatment of diabetic retinopathy based on_network pharmacology. BMC Complementary Medicine and Therapies. 2022 Dec;22(1):1-12Read more>>
  • Jiakun Dai. et al. Multimeric adiponectin nanoparticles regulate glucose metabolism by activating phosphatidylinositol-3-kinase, protein kinase B and T-cadherin. PROCESS BIOCHEM. 2022 DecRead more>>