DATASHEET
Host:
Mouse
Target Protein:
ERK1/2
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
5595
Swiss Prot:
P27361
Source:
KLH conjugated synthetic peptide derived from human ERK1/2
Purification:
Purified by Protein G.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
PRODUCT SPECIFIC PUBLICATIONS
- Jialei Tian. et al. Chondroitin sulphate modified MoS2 nanoenzyme with multifunctional activities for treatment of Alzheimer's disease. INT J BIOL MACROMOL. 2024 May;266:131425Read more>>
- Xiaoxiang Hu. et al. The suppressive role of NLRP6 in host defense against Streptococcus suis infection. VET MICROBIOL. 2024 Jun;:110166Read more>>
- Lan Yan-Ping. et al. Analysis of the functional role and mRNA expression of GABABR in the nucleus accumbens of cocaine-addicted rats. J CHIN MED ASSOC. 2024 Jun;:10.1097/JCMA.0000000000001119Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:200 overnight at 4°C, DAB staining.