Host: Mouse
Target Protein: GRP78/Bip
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 3309
Swiss Prot: P11021
Source: KLH conjugated synthetic peptide derived from human GRP78/Bip
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 78
Cross Reactive Species: Human
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
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HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GRP78/Bip) monoclonol Antibody, Unconjugated (bsm-33224M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.