DATASHEET
Host:
Mouse
Target Protein:
GRP78/Bip
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
3309
Swiss Prot:
P11021
Source:
KLH conjugated synthetic peptide derived from human GRP78/Bip
Purification:
Purified by Protein G.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
PRODUCT SPECIFIC PUBLICATIONS
- Jianzhao Liao. et al. Endoplasmic Reticulum Stress Contributes to Copper-Induced Pyroptosis via Regulating the IRE1_-XBP1 Pathway in Pig Jejunal Epithelial Cells. J Agr Food Chem. 2022;70(4):12931303Read more>>
- Dajin Pi. et al. Tanshinone IIA Inhibits the Endoplasmic Reticulum Stress-Induced Unfolded Protein Response by Activating the PPAR/FGF21 Axis to Ameliorate Nonalcoholic Steatohepatitis. ANTIOXIDANTS-BASEL. 2024 Sep;13(9):1026Read more>>
VALIDATION IMAGES
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GRP78/Bip) monoclonol Antibody, Unconjugated (bsm-33221M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.