bsm-33196M [Primary Antibody]
CREB1 (6B4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: CREB1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1385

Swiss Prot: P16220

Source: Recombinant human CREB1 protein

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tang Q et al. Aqueous extract from You-Gui-Yin ameliorates cognitive impairment of chronic renal failure micethrough targeting hippocampal CaMKIIα/CREB/BDNF and EPO/EPOR pathways. J Ethnopharmacol. 2019 Jul 15;239:111925. Read more>>
VALIDATION IMAGES

NIH/3T3 cell lysates probed with CREB1 (6B4) Monoclonal Antibody, unconjugated (bsm-33196M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Human Jurkat cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human U-2OS cell lysates probed with CREB1 (6B4) Monoclonal Antibody, Unconjugated (bsm-33196M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.