bsm-33190M
[Primary Antibody]
Caspase 8 Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: Caspase 8
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 841
Swiss Prot: Q14790
Source: Recombinant human Caspase 8 Protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Xu B et al. Excessive mechanical stress induces chondrocyte apoptosis through TRPV4 in an anteriorcruciate ligament-transected rat osteoarthritis model. Life Sci. 2019 Jul 1;228:158-166. Read more>>
- Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639. Read more>>
- Zhao W et al. Junduqing extractive promotes the apoptosis of nasopharyngeal carcinoma cells through down-regulating Mcl-1 and Bcl-xL and up-regulating Caspase-3, Caspase-8 and Caspase-9. Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3904-3912.Read more>>
- Lei Xiang. et al. Antitumor effects of curcumin on the proliferation, migration and apoptosis of human colorectal carcinoma HCT?116 cells. Oncol Rep. 2020 Nov;44(5):1997-2008Read more>>
- Peng Zheng. et al. Alleviative effect of melatonin on the decrease of uterine receptivity caused by blood ammonia through ROS/NF-_B pathway in dairy cow. Ecotox Environ Safe. 2022 Feb;231:113166Read more>>
- Rentao Zhang. et al. Anti-Gastric Cancer Activity of the Cell-free Culture Supernatant of Serofluid Dish and Lactiplantibacillus plantarum YT013. FRONT BIOENG BIOTECH. 2022; 10: 89824Read more>>
- Min-Lin He. et al. Screening and characterization of a novel efficient tumor cell-targeting peptide derived from insulin-like growth factor binding proteins. J DRUG TARGET. 2023 Mar 28Read more>>
- Rentao Zhang. et al. Exopolysaccharide from Lactiplantibacillus plantarum YT013 and Its Apoptotic Activity on Gastric Cancer AGS Cells. Fermentation-Basel. 2023 Jun;9(6):539Read more>>
- Hai-bo Liu. et al. Dispelling Dampness, Relieving Turbidity and Dredging Collaterals Decoction, Attenuates Potassium Oxonate-Induced Hyperuricemia in Rat Models. DRUG DES DEV THER. 2023 Aug 01Read more>>
- Gan Rao. et al. Arsenic and polystyrene-nano plastics co-exposure induced testicular toxicity: Triggers oxidative stress and promotes apoptosis and inflammation in mice. ENVIRON TOXICOL. 2023 SepRead more>>
- Shuli Yang. et al. Enrichment and Evaluation of Antitumor Properties of Total Flavonoids from Juglans mandshurica Maxim. MOLECULES. 2024 Jan;29(9):1976Read more>>
- o Fang. et al. Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model. BIOMOLECULES. 2024 Aug;14(8):901Read more>>
VALIDATION IMAGES
Lane 1: hela lysates; Lane 2: jurkat lysates probed with Caspase 8 Monoclonal Polyclonal Antibody, Unconjugated (bs-33190M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: 293T-UV cell lysates; Lane 2: U251-UV cell lysates probed with Caspase 8 Monoclonal Antibody, Unconjugated (bsm-33190M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Caspase 8 Monoclonal Antibody, Unconjugated (bsm-33190M) at 1:2000 overnight at 4°C, DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human MOLT-4 cell lysates; Lane 4: Human K562 cell lysates probed with Caspase 8 Monoclonal Antibody, Unconjugated (bsm-33190M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Stomach lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse Lymph node lysates; Lane 4: Mouse Urinary bladder lysates probed with Caspase 8 Monoclonal Antibody, Unconjugated (bsm-33190M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.