bsm-33185M [Primary Antibody]
EBFP-Tag(7B12) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Enhanced Blue Fluorescent Protein

Clonality: Monoclonal

Isotype: IgG

Source: Recombinant EBFP protein

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Enhanced Blue Fluorescent Protein (EBFP) emits a strong blue fluorescence. EBFP can be used as protein "tags" to study the sub-cellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 26kDa


Cross Reactive Species: Others

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: EBFP-Tagged fusion protein (full length) Overexpression E.coli lysates (Cat#: bs-33185P);Lane 2: Control (-) E.coli lysates probed with EBFP-Tag Monoclonal Antibody, Unconjugated (bsm-33185M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.