Host: Mouse
Target Protein: Vimentin
Specificity: KO-Validated
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 7431
Swiss Prot: P08670
Source: Recombinant human Vimentin Protein
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications: WB(1:300-5000)
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
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- Juan-Juan Zhu. et al. VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation. Front Cell Dev Biol. 2020; 8: 576826Read more>>
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- Jianan Zhao. et al. DNMT1 mediates the disturbed flow-induced endothelial to mesenchymal transition through disrupting -alanine and carnosine homeostasis. THERANOSTICS. 2023; 13(13): 4392C4411Read more>>
- Chen Si-Yu. et al. miR-324-3p Suppresses Hepatic Stellate Cell Activation and Hepatic Fibrosis Via Regulating SMAD4 Signaling Pathway. MOL BIOTECHNOL. 2024 Feb;:1-16Read more>>
Lane 1: Raji; Lane 2: U87MG cell lysate probed with Vimentin (3B7) Monoclonal Antibody, unconjugated (bsm-33170M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Lane 1: HeLa (30ug); Lane 2: HeLa KO Vimentin lysate (30ug) probed with Vimentin (3B7) Monoclonal Antibody, unconjugated (bsm-33170M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
25 ug total protein per lane of various lysates (see on figure) probed with Vimentin monoclonal antibody, unconjugated (bsm-33170M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.