Host: Mouse
Target Protein: IL2RA/CD25
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 3559
Swiss Prot: P01589
Source: KLH conjugated synthetic peptide derived from human IL2RA/CD25
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Size: 100ul
Concentration: 1mg/ml
Applications: FCM(1:100)
Predicted Molecular Weight: 28
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
scatter diagram showing peripheral blood lymphocytes stained with CD25. The cells were incubated with the antibody (bsm-33113M) for 30 min at 22°C.The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.
SR cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD25 .Antibody(bsm-33113M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).