DATASHEET
Host:
Mouse
Target Protein:
Histone H3 (tri methyl K4)
Modification Site:
K4
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
8290
Swiss Prot:
P68431
Source:
KLH conjugated synthetic peptide derived from human Histone H3 (tri methyl K4)
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
PRODUCT SPECIFIC PUBLICATIONS
- Liu, Yan. et al. Sexual dimorphism of DNA and histone methylation profiles in the gonads of the olive flounder Paralichthys olivaceus. 2021 Jul 15Read more>>
- Cui Guina. et al. WD repeat domain 82 (Wdr82) facilitates mouse iPSCs generation by interfering mitochondrial oxidative phosphorylation and glycolysis. CELL MOL LIFE SCI. 2023 Aug;80(8):1-15Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Histone H3 (tri methyl K4) Monoclonal Antibody, Unconjugated (bsm-33110M) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (tri methyl K4)) Monoclonal Antibody, Unconjugated (bsm-33110M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.