bsm-33070M [Primary Antibody]
Ki-67 Monoclonal Antibody
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DATASHEET

Host: Mouse

Target Protein: Ki-67

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4288

Swiss Prot: P46013

Source: KLH conjugated synthetic peptide derived from human Ki-67

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()

Predicted Molecular Weight: 358 kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang et al. Effects of Neuropeptide Substance P on Proliferation and β-Cell Differentiation of Adult Pancreatic Ductal Cells. (2018) Front.Neurosci. 12:808Read more>>
  • Peng W et al. Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onsetpreeclampsia. FASEB J. 2019 Feb 18:fj201802058RR. Read more>>
  • Du Y et al. ATPR triggers acute myeloid leukaemia cells differentiation and cycle arrest via the RAR/LDHB/ERK\glycolysis signalling axis. J Cell Mol Med. 2020 Jun;24(12):6952-6965.Read more>>
  • Jian-Bo Lin et al. Involvement of activin a receptor type 1 (ACVR1) in the pathogenesis of primary focal hyperhidrosis. Biochem Biophys Res Commun. 2020 Jul 23;528(2):299-304.Read more>>
  • Jia Fangyuan. et al. BOP1 Knockdown Attenuates Neointimal Hyperplasia by Activating p53 and Inhibiting Nascent Protein Synthesis. Oxid Med Cell Longev. 2021;2021:598626Read more>>
  • Zhang, Yichao. et al. STK25 enhances hepatocellular carcinoma progression through the STRN/AMPK/ACC1 pathway. Cancer Cell Int. 2022 Dec;22(1):1-23Read more>>
  • Yun Wang. et al. Prucalopride might improve intestinal motility by promoting the regeneration of the enteric nervous system in diabetic rats. INT J MOL MED. 2022 Jul;50(1):1-1Read more>>
  • Gu Min. et al. Inhibition of PIKfyve Ameliorates the Proliferation and Migration of Vascular Smooth Muscle Cells and Vascular Intima Hyperplasia By Reducing mTORC1 Activity. J CARDIOVASC PHARM. 2022 May;79(5):739-748Read more>>
  • Yongxin Guo. et al. Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis. INT J MOL SCI. 2022 Jan;23(21):13152Read more>>
  • Lei Li. et al. Carrier-Free Nanoplatform via Evoking Pyroptosis and Immune Response against Breast Cancer. ACS APPL MATER INTER. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Gao Yanan. et al. Resveratrol alleviates oxidative stress induced by oxidized soybean oil and improves gut function via changing gut microbiota in weaned piglets. Journal of Animal Science and Biotechnology. 2023 Dec;14(1):1-22Read more>>
  • Xirui Duan. et al. Photothermal-Starvation Therapy Nanomodulator Capable of Inhibiting Colorectal Cancer Recurrence and Metastasis by Energy Metabolism Reduction. ADV HEALTHC MATER. 2023 Aug;:2300968Read more>>
  • Huang Bo. et al. A novel peptide PDHK1-241aa encoded by circPDHK1 promotes ccRCC progression via interacting with PPP1CA to inhibit AKT dephosphorylation and activate the AKT-mTOR signaling pathway. MOL CANCER. 2024 Dec;23(1):1-27Read more>>
  • Zhenzhen Yang. et al. Reinforced Immunogenic Endoplasmic Reticulum Stress and Oxidative Stress via an Orchestrated Nanophotoinducer to Boost Cancer Photoimmunotherapy. ACS NANO. 2024;XXXX(XXX):XXX-XXXRead more>>
  • Shuhong Kang. et al. Hsa_circ_0008133 contributes to lung cancer progression by promoting glycolysis metabolism through the miR-760/MEX3A axis. ENVIRON TOXICOL. 2024 FebRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Ki-67 Monoclonal Antibody, Unconjugated (bsm-33070M) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Ki-67 Monoclonal Antibody, Unconjugated (bsm-33070M) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human cecum cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CK7 Monoclonal Antibody, Unconjugated (bsm-33070M) at 1:200 overnight at 4°C, DAB staining.


Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Ki-67 Antibody(bsm-33070M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).