bsm-33042M
[Primary Antibody]
Histone H3/HIST3H3 (3G1) Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: Histone H3
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 8290
Swiss Prot: Q16695
Source: Recombinant human HIST3H3 Protein
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Tian, Yue, et al. "Minocycline attenuates sevoflurane-induced cell injury via activation of Nrf2." International Journal of Molecular Medicine.Read more>>
- Zhao, Dongmei, et al. "Unfractionated heparin protects the protein C system against lipopolysaccharide‑induced damage in vivo and in vitro." Experimental and Therapeutic Medicine.Read more>>
- Zhao et al. Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damagein vivoandin vitro. (2017) Exp.Ther.Med. 14:5515-5522Read more>>
- Dong R et al. Temporospatial effects of acyl‐ghrelin on activation of astrocytes after ischemic brain injury. J Neuroendocrinol. 2019 Jul 5:e12767. Read more>>
- Zheng GH et al. Purple Sweet Potato Color Attenuates Kidney Damage by Blocking VEGFR2/ROS/NLRP3 Signaling in High-Fat Diet-Treated Mice. Oxid Med Cell Longev. 2019 Jan 22;2019:5189819. Read more>>
- Adil Hassan et al. Anti-atherosclerotic effects of Lactobacillus plantarum ATCC 14917 in ApoE ?/? mice through modulation of proinflammatory cytokines and oxidative stress. Appl Microbiol Biotechnol. 2020 Jul;104(14):6337-6350.Read more>>
- Lei Chen. et al. Perillaldehyde: A promising antifungal agent to treat oropharyngeal candidiasis. Biochem Pharmacol. 2020 Oct;180:114201Read more>>
- Qing Shu. et al. Mechanisms by which electroacupuncture?mediated histone acetylation mitigates bone loss in rats with ovariectomy?induced osteoporosis. Mol Med Rep. 2020 Oct;22(4):3453-3463Read more>>
- Chu Xuan. et al. DNMT3A R882H mutation drives daunorubicin resistance in acute myeloid leukemia via regulating NRF2/NQO1 pathway. CELL COMMUN SIGNAL. 2022 Dec;20(1):1-14Read more>>
- Hongying Dong. et al. Pueraria lobata antioxidant extract ameliorates non-alcoholic fatty liver by altering hepatic fat accumulation and oxidative stress. J ETHNOPHARMACOL. 2024 Oct;333:118468Read more>>
VALIDATION IMAGES
HeLa cell lysates; probed with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
K562 cell lysates; probed with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: NIH/3T3 cell lysates probed with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Large intestine lysates probed with Histone H3/HIST3H3 Monoclonal Antibody, Unconjugated (bsm-33042R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Small intestine lysates probed with Histone H3/HIST3H3 Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Large intestine lysates; Lane 2: Human MCF-7 cell lysates; Lane 3:Human U-2OS cell lysates probed with Histone H3/HIST3H3 Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3/HIST3H3 (3G1)) Monoclonal Antibody, Unconjugated (bsm-33042M ) at 1:500 overnight at 4°C, followed by a conjugated Goat Anti- Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3/HIST3H3 (3G1)) Monoclonal Antibody, Unconjugated (bsm-33042M ) at 1:500 overnight at 4°C, followed by a conjugated Goat Anti- Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3/HIST3H3 (3G1)) Monoclonal Antibody, Unconjugated (bsm-33042M ) at 1:500 overnight at 4°C, followed by a conjugated Goat Anti- Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 /HIST3H3 (3G1)) Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embeddedHuman Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of bsm-33042M) at 1:1500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with Histone H3 monoclonal antibody, unconjugated (bsm-33042M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.