bsm-33039M [Primary Antibody]
alpha Tubulin Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: alpha Tubulin

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7277

Swiss Prot: P68366

Source: Recombinant human alpha Tubulin Protein

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yaying Wang. et al. Transcriptome sequencing reveals differences between leydig cells and sertoli cells of yak.. FRONT VET SCI. 2022 Aug;9:960250-96025Read more>>
  • Jinglei Wang. et al. Follicular fluid exosomes regulate OVGP1 secretion in yak oviduct epithelial cells via autophagy in vitro. J CELL PHYSIOL. 2023 ApRead more>>
  • Yingjun Zhou. et al. Auricularia auricula-judae (Bull.) polysaccharides improve obesity in mice by regulating gut microbiota and TLR4/JNK signaling pathway. INT J BIOL MACROMOL. 2023 Oct;250:126172Read more>>
  • Junling Gao. et al. Mechanisms underlying the synergistic effects of chuanxiong combined with Chishao on treating acute lung injury based on network pharmacology and molecular docking combined with preclinical evaluation. J ETHNOPHARMACOL. 2024 May;325:117862Read more>>
  • Sibo Wang. et al. Design, synthesis and biological evaluation of novel tubulin-targeting agents with a dual-mechanism for polymerization inhibition and protein degradation. EUR J MED CHEM. 2024 Jun;272:116458Read more>>
VALIDATION IMAGES

Lane 1: Mouse Lung lysates; Lane 2: Hela cell lysates; Lane 3: U87MG cell lysates; Lane 4: Mouse Uterus lysates probed with alpha Tubulin Monoclonal Antibody, Unconjugated (bsm-33039M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human Hela cell Lysates; Lane 2: Human Jurkat cell Lysates; Lane 3: Human SH-SY5Y cell Lysates; Lane 4: Human U-2 OS cell Lysates; Lane 5: Human A431 cell Lysates; Lane 6: Human A549 cell Lysates. Probed with Alpha Tubulin monoclonal Antibody, unconjugated (bsm-33039M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human A431 cell lysates; Lane 7: Human U251 cell lysates; Lane 8: Human 293T cell lysates; Lane 9: Human HepG2 cell lysates probed with alpha Tubulin Monoclonal Antibody, Unconjugated (bsm-33039M) at 1:100000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.