bsm-33035M-HRP [Conjugated Primary Antibody]
PCNA (3G1) Monoclonal Antibody, HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: PCNA

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5111

Swiss Prot: P12004

Source: Recombinant human PCNA protein

Purification: Purified by Protein G.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xu Hongyan. et al. The New Roles of traf6Gene Involved in the Development of Zebrafish Liver and Gonads. MAR BIOTECHNOL. 2024 Jun;:1-14Read more>>
VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with PCNA monoclonal antibody, HRP conjugated (bsm-33035M-HRP) at 1:1000 dilution and 4°C overnight incubation.


Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Speleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Speleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4°C, followed by DAB (C-0010) staining.