bsm-33019R
[Primary Antibody]
GFP Recombinant Antibody
DATASHEET
Host: Rabbit
Target Protein: GFP
Clonality: Recombinant
Isotype: N/A
Source: Recombinant Green Fluorescence Protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin
Size: 100ul
Concentration: 1ug/ul
Applications: WB(1:300-5000)
Cross Reactive Species: Belt Jellyfish
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
20 ng rGFP-His protein (bs-33009P) per lane probed with GFP monoclonal antibody respectively, unconjugated (bsm-33019R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
50 ng rGFP-His protein (bs-33009P) per lane probed with GFP monoclonal antibody respectively, unconjugated (bsm-33019R) at 1:10000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.