bsm-33004M-HRP [Conjugated Primary Antibody]
His tag (6E6) Monoclonal Antibody, HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: His tag

Clonality: Monoclonal

Isotype: IgG

Source: Human, fusion protein HHHHHH tagged

Purification: Purified by Protein G.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Rong Yue. et al. Study of the Effects of Several SARS-CoV-2 Structural Proteins on Antiviral Immunity. VACCINES-BASEL. 2023 Mar;11(3):524Read more>>
  • eibei Wang. et al. Identification and functional analysis of Toll-like receptor 2 from razor clam Sinonovacula constricta. INT J BIOL MACROMOL. 2024 Apr;265:131029Read more>>
VALIDATION IMAGES

50 ng rGFP-His protein (bs-33009P) per lane probed with His tag monoclonal antibody respectively, HRP conjugated (bsm-33004M-HRP) at 1:100000 dilution and 4°C overnight incubation.