bsm-33004M
[Primary Antibody]
His tag (6E6) Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: His tag
Clonality: Monoclonal
Isotype: IgG
Source: Human, fusion protein HHHHHH tagged
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Size: 100ul
Concentration: 1ug/ul
Applications: WB(1:300-5000)
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Rong Yue. et al. Study of the Effects of Several SARS-CoV-2 Structural Proteins on Antiviral Immunity. VACCINES-BASEL. 2023 Mar;11(3):524Read more>>
- eibei Wang. et al. Identification and functional analysis of Toll-like receptor 2 from razor clam Sinonovacula constricta. INT J BIOL MACROMOL. 2024 Apr;265:131029Read more>>
VALIDATION IMAGES
Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate; Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate probed with His tag (6E6) Monoclonal Antibody, Unconjugated (bsm-33004M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (bs- 41230P) probed with His tag Monoclonal Antibody, Unconjugated (bsm-33004M) at 1:200000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.