bsm-10895M
[Primary Antibody]
TIMP-1 (4C2) Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: TIMP-1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 7076
Swiss Prot: P01033
Source: Recombinant human TIMP-1
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq].
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 21 kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- M Li. et al. Histology\based profile of inflammatory mediators in experimentally induced pulpitis in a rat model: screening for possible biomarkers. 2021 Mar 14Read more>>
- Yipeng Wan. et al. Ursolic acid alleviates Kupffer cells pyroptosis in liver fibrosis by the NOX2/NLRP3 inflammasome signaling pathway. INT IMMUNOPHARMACOL. 2022 Dec;113:109321Read more>>
- Huiyuan Zhu. et al. RIG-I contributes to keratinocyte proliferation and wound repair by inducing TIMP-1 expression through NF-B signaling pathway. J CELL PHYSIOL. 2023 JuRead more>>
- Wen-Hai Zhuo. et al. Increased matrix metalloproteinase-2 in ligamentum flavum hypertrophy and the regulation of MMP-2/TIMPs by elastin-derived peptides. J ORTHOP RES. 2024 MaRead more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TIMP-1 (4C2) Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TIMP-1 (4C2)Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TIMP-1 (4C2)Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TIMP-1 (4C2)Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4°C, DAB staining.
Lane 1: Recombinant Human TIMP-1 Protein; Probed with TIMP-1 Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:50000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Human Brain Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TIMP-1 Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4°C, followed by a FITC-conjugated Goat Anti-Mouse IgG antibody (bs-0296G-FITC) for 90 minutes.
Lane 1: Mouse Stomach tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Rat Stomach tissue lysates; Lane 4: Rat Colon tissue lysates; Lane 5: Rat Lung tissue lysates probed with TIMP-1 Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.