bsm-0978M [Primary Antibody]
GAPDH(3E12) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: GAPDH

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2597

Swiss Prot: P04406

Source: full length GAPDH protein

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 36


Cross Reactive Species: Human
Mouse
Rat
Sheep
Rabbit
Monkey

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Kang, Hongyan, et al. "A Potential Gravity-Sensing Role of Vascular Smooth Muscle Cell Glycocalyx in Altered Gravitational Stimulation." Astrobiology (2013).Read more>>
  • Wei, Ting, et al. "The role of N-acetylglucosaminyltransferases V in the malignancy of human hepatocellular carcinoma." Experimental and molecular pathology (2012).Read more>>
  • Zhang, Xiao‐Qun, et al. "Regulation of pulmonary surfactant synthesis in fetal rat type II alveolar epithelial cells by microRNA‐26a." Pediatric Pulmonology (2014).Read more>>
  • Zhao, Tianyun, et al. "Ketamine administered to pregnant rats in the second trimester causes long-lasting behavioral disorders in offspring." Neurobiology of Disease (2014).Read more>>
  • Xu, Xiaochun, et al. "PPARγ, FAS, HSL mRNA and protein expression during Tan sheep fat-tail development." Electronic Journal of Biotechnology (2015).Read more>>
  • Xu, Xiaochun, et al. "mRNA transcription and protein expression of PPARγ, FAS, and HSL in different parts of the carcass between fat-tailed and thin-tailed sheep." Electronic Journal of Biotechnology (2015).Read more>>
  • Cui, Yanjun, and Xianhong Gu. "Proteomic changes of the porcine small intestine in response to chronic heat stress." Journal of Molecular Endocrinology (2015): JME-15.Read more>>
  • Liu, Yong, et al. "Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model." Experimental and Therapeutic Medicine.Read more>>
  • Liu, Si-jun, et al. "Neuroprotective effect of β-asarone against Alzheimer’s disease: regulation of synaptic plasticity by increased expression of SYP and GluR1." Drug design, development and therapy 10 (2016): 1461.Read more>>
  • Di, Yu, et al. "CCN1/Cyr61-PI3K/AKT signaling promotes retinal neovascularization in oxygen-induced retinopathy." International journal of molecular medicine 36.6 (2015): 1507-1518.Read more>>
  • Pei, Haitao, et al. "The Effect of Minimally Invasive Hematoma Aspiration on the JNK Signal Transduction Pathway after Experimental Intracerebral Hemorrhage in Rats." International journal of molecular sciences 17.5 (2016): 710.Read more>>
  • Katoh, Hiroshi, et al. "Heat shock protein 90 ensures efficient mumps virus replication by assisting with viral polymerase complex formation." Journal of Virology (2017): JVI-02220.Read more>>
  • Fahie, Monifa, "Pore forming protein assembly and the use in nanopore sensing: a study on E. coli proteins ClyA and OmpG" (2017). Doctoral Dissertations. 1076.Read more>>
  • Fahie, Monifa, "Pore forming protein assembly and the use in nanopore sensing: a study on E. coli proteins ClyA and OmpG" (2017). Doctoral Dissertations. 1076.Read more>>
  • Usuki, Seigo, et al. "Characterization of Konjac Ceramide (kCer) Binding to Sema3A Receptor Nrp1." Journal of oleo science (2017): ess17142.Read more>>
  • Zhou et al. miR612 is associated with esophageal squamous cell carcinoma development and metastasis, mediated through TP53. (2017) Mol.Med.Re. 16:1855-1863Read more>>
  • Liu et al. Inhibition of Expression of the S100A8 Gene Encoding the S100 Calcium-Binding Protein A8 Promotes Apoptosis by Suppressing the Phosphorylation of Protein Kinase B (Akt) in Endometrial Carcinoma and HEC-1A Cells. (2018) Med.Sci.Monit. 24:1836-1846Read more>>
  • Kim et al. Relaxin Receptor RXFP1 and RXFP2 Expression in Ligament, Tendon, and Shoulder Joint Capsule of Rats. (2016) J.Korean.Med.Sci. 31:983-8Read more>>
  • Wang YN et al. Expression of visual system homeobox 1 in human keratoconus. Int J Ophthalmol. 2019 Feb 18;12(2):201-206.Read more>>
  • Usuki S et al. Konjac ceramide (kCer) regulates keratinocyte migration by Sema3A-like repulsion mechanism. Biochem Biophys Rep. 2019 Jan 3;17:132-138. Read more>>
  • Gao J et al. Proteomic Analyses of Mammary Glands Provide Insight into the Immunity and Metabolism Pathways Associated with Clinical Mastitis in Meat Sheep.Animals (Basel). 2019 May 31;9(6). pii: E309.Read more>>
  • Ma WJ et al. Biliary antibiotics irrigation for E. coli-induced chronic proliferative cholangitis and hepatolithiasis: A pathophysiological study in rabbits. Clin Res Hepatol Gastroenterol. 2019 Aug 13. pii: S2210-7401(19)30173-1.Read more>>
  • Wang F et al. Degradation of CCNB1 mediated by APC11 through UBA52 ubiquitination promotes cell cycle progression and proliferation of non-small cell lung cancer cells. Am J Transl Res. 2019 Nov 15;11(11):7166-7185.Read more>>
  • Hu N et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. J Ethnopharmacol. 2019 Nov 1:112361.Read more>>
  • Qu S et al. Cinnamaldehyde, a Promising Natural Preservative Against Aspergillus flavus. Front Microbiol. 2019 Dec 18;10:2895. Read more>>
  • Jin Y et al. Cancer-associated mutation abolishes the impact of TRIM21 on the invasion of breast cancer cells. Int J Biol Macromol. 2019 Oct 14. pii: S0141-8130(19)34947-5.Read more>>
  • Zhang W et al. Cinnamaldehyde Enhances Antimelanoma Activity through Covalently Binding ENO1 and Exhibits a Promoting Effect with Dacarbazine. Cancers (Basel). 2020 Jan 29;12(2). pii: E311.Read more>>
  • Shuhai Heet al. Avian leukosis virus subgroup J induces B cell anergy mediated by Lyn inhibited BCR signal transduction. Vet Microbiol. 2020 Aug;247:108781.Read more>>
  • Cong Wanget al. Disruption of FGF Signaling Ameliorates Inflammatory Response in Hepatic Stellate Cells. Front Cell Dev Biol. 2020 Jul 22;8:601.Read more>>
  • Xia Qinet al. Zinc Oxide Nanoparticles Induce Ferroptotic Neuronal Cell Death in vitro and in vivo. Int J Nanomedicine. 2020 Jul 27;15:5299-5315.Read more>>
  • Di Xia et al. Nrf2 promotes esophageal squamous cell carcinoma (ESCC) resistance to radiotherapy through the CaMKII-associated activation of autophagy. Cell Biosci. 2020 Jul 30;10:90.Read more>>
  • obae Kimet al. B-Cell-Activating Factor Depletion Ameliorates Aging-Dependent Insulin Resistance via Enhancement of Thermogenesis in Adipose Tissues. Int J Mol Sci. 2020 Jul 20;21(14):5121.Read more>>
  • Weiyi Zhanget al. Cinnamaldehyde changes the dynamic balance of glucose metabolism by targeting ENO1. Life Sci. 2020 Oct 1;258:118151.Read more>>
  • Xuesong Wanget al. LncRNA NEAT1 Regulates 5-Fu Sensitivity, Apoptosis and Invasion in Colorectal Cancer Through the MiR-150-5p/CPSF4 Axis. Onco Targets Ther. 2020 Jul 1;13:6373-6383.Read more>>
  • Ming Lyuet al. Overexpression of the Tuberous sclerosis complex 2 (TSC2) gene inhibits Goat myoblasts proliferation and differentiation in understanding the underlying mechanism of muscle development. Gene. 2020 Oct 5;757:144943.Read more>>
  • Chen T et al. p53 Mediates GnRH Secretion via Lin28/let-7 System in GT1-7 CellsDiabetes Metab Syndr Obes.2020 Dec 1;13:4681-4688.Read more>>
  • Gaolong Zhong. et al. Arsenic or/and antimony induced mitophagy and apoptosis associated with metabolic abnormalities and oxidative stress in the liver of mice. Sci Total Environ. 2021 Jul;777:146082Read more>>
  • Seona Cho. et al. Viburnum stellato-tomentosum Extract Suppresses Obesity and Hyperglycemia through Regulation of Lipid Metabolism in High-Fat Diet-Fed Mice. Molecules. 2021 Jan;26(4):1052Read more>>
  • Gaolong Zhong. et al. The protective role of autophagy against arsenic trioxide-induced cytotoxicity and ROS-dependent pyroptosis in NCTC-1469 cells. J Inorg Biochem. 2021 Apr;217:111396Read more>>
  • Xiao-Yi Zhang. et al. Dietary capsaicin normalizes CGRP peptidergic DRG neurons in experimental diabetic peripheral neuropathy. Sci Rep-Uk. 2021 Jan;11(1):1-19Read more>>
  • Hongyi Qu. et al. The effects of vasoactive intestinal peptide on RANKL-induced osteoclast formation. Ann Transl Med. 2021 Jan; 9(2): 127Read more>>
  • Yongtao Li. et al. Effects of epigallocatechin gallate (EGCG) on the biological properties of human dental pulp stem cells and inflammatory pulp tissue. Arch Oral Biol. 2021 Mar;123:105034Read more>>
  • Yukun Fang. et al. Activation of the ROS/HO-1/NQO1 signaling pathway contributes to the copper-induced oxidative stress and autophagy in duck renal tubular epithelial cells. Sci Total Environ. 2021 Feb;757:143753Read more>>
  • Shuai Chen. et al. Tenogenic adipose-derived stem cell sheets with nanoyarn scaffolds for tendon regeneration. Mat Sci Eng C-Mater. 2021 Feb;119:111506Read more>>
  • Ting Chen. et al. Overexpression of p53 accelerates puberty in high-fat dietCfed mice through Lin28/let-7 system:. Exp Biol Med. 2020;246(1):66-71Read more>>
  • Ji-Lun Cheng. et al. LncRNA KCNQ1OT1 regulates the invasion and migration of hepatocellular carcinoma by acting on S1PR1 through miR-149. Cancer Gene Ther. 2020 Aug;:1-14Read more>>
  • Cuie Gao. et al. Pathogenic role of circulating CD4+CXCR5+ cell subpopulations in patients with chronic spontaneous urticarial.. Am J Transl Res. 2020; 12(8): 4434C4444Read more>>
  • Luo Ping. et al. IL-37 inhibits M1-like macrophage activation to ameliorate temporomandibular joint inflammation through the NLRP3 pathway. Rheumatology. 2020 Oct;59(10):3070-308Read more>>
  • Guo Qiang. et al. PAQR3 Inhibits Non-small Cell Lung Cancer Growth by Regulating the NF-B/p53/Bax Axis. Front Cell Dev Biol. 2020 Oct;8:106Read more>>
  • Yuntai Shen. et al. Myricitrin pretreatment ameliorates mouse liver ischemia reperfusion injury. Int Immunopharmacol. 2020 Dec;89:107005Read more>>
VALIDATION IMAGES

Human HepG2 cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Human A549 cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Lane 1: mouse placenta; Lane 2: 293T cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Lane 1: A549 cell lysates; Lane 2: 293T cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Hela cell lysates probed with GAPDH(3E12) Monoclonal Antibody, Unconjugated (bsm-0978M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: A549 cell lysates; Lane 2: 293T cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Hela cell lysates probed with GAPDH(3E12) Monoclonal Antibody, Unconjugated (bsm-0978M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human Hela cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human MCF-7 cell lysates probed with GAPDH Monoclonal Antibody, Unconjugated (bsm-0978M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.