DATASHEET
Host:
Rabbit
Target Protein:
LEF1 Ser42
Specificity:
This antibody will recognize LEF1(Ser40) in mouse and rat
Modification Site:
Ser42
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
51176
Swiss Prot:
Q9UJU2
Source:
KLH conjugated synthetic phosphopeptide derived from human LEF1 around the phosphorylation site of Ser42
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
PRODUCT SPECIFIC PUBLICATIONS
- Fu, Qiang, et al. "Proteome Profile and Quantitative Proteomic Analysis of Buffalo (Bubalusbubalis) Follicular Fluid during Follicle Development."International Journal of Molecular Sciences 17.5 (2016): 618.Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-phospho-LEF1(Ser42) Polyclonal Antibody, Unconjugated (bs-9842R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: HL60 cell lysates probed with LEF1(Ser42) Polyclonal Antibody, Unconjugated (bs-9842R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Spleen tissue lysates probed with phospho-LEF1 (Ser42) Polyclonal Antibody, Unconjugated (bs-9842R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.