bs-9743R [Primary Antibody]
ANKLE2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
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DATASHEET

Host: Rabbit

Target Protein: ANKLE2

Immunogen Range: 251-350/938


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23141

Swiss Prot: Q86XL3

Source: KLH conjugated synthetic peptide derived from human ANKLE2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. Mutations of ankyrin genes lead to severe genetic diseases such as fatal cardiac arrhythmias and hereditary spherocytosis. ANKLE2 (ankyrin repeat and LEM domain containing 2), also known as LEMD7, is a 938 amino acid single-pass membrane protein containing an ANK repeat and a LEM domain. Exsiting as two isoforms produced by alternative splicing events, the gene encoding ANKLE2 maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and trisomy 12p, which causes facial developmental defects and seizure disorders.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 104


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human HepG2 cell lysates; Lane 2: Human MOLT-4 cell lysates; Lane 3: Human HL-60 cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human A549 cell lysates; Lane 6: Mouse Cerebrum lysates; Lane 7: Mouse Stomach lysates; Lane 8: Mouse Lung lysates; Lane 9: Mouse Spleen lysates; Lane 10: Mouse Heart lysates probed with ANKLE2 Polyclonal Antibody, Unconjugated (bs-9743R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.