bs-9606R [Primary Antibody]
RBM20 Polyclonal Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RBM20

Immunogen Range: 361-460/1227


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 282996

Swiss Prot: Q5T481

Source: KLH conjugated synthetic peptide derived from human RBM20

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes involved in cardiac development. Regulates splicing of TTN (Titin).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
Flow-Cyt(Flow-Cyt=1ug/test)

Predicted Molecular Weight: 134


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat heart labeled with Anti-RBM20 Polyclonal Antibody, Unconjugated (bs-9606R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: K562 cell lysates; Lane 2: A549 cell lysates probed with RBM20 Polyclonal Antibody, Unconjugated (bs-9606R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.