DATASHEET
Host:
Rabbit
Target Protein:
Factor H
Immunogen Range:
621-710/1231
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
3075
Swiss Prot:
P08603
Source:
KLH conjugated synthetic peptide derived from human Factor H
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.
PRODUCT SPECIFIC PUBLICATIONS
- Wang,et al.Screening of genes involved in epithelial-mesenchymal transition and differential expression of complement-related genes induced by PAX2 in renal tubules.() Nephrology. :.Read more>>
- Zhang,et al.Effect of local scrotal heating on the expression of tight junction-associated molecule Occludin in boar testes.(2018) Reproduction in Domestic Animals. 53:458-462.Read more>>
- Chen C et al. Alternative complement pathway is activated in the brains of scrapie-infected rodents. Med Microbiol Immunol. 2019 Nov 12.Read more>>
- ingsheng Yang. et al. Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma. Oncol Lett. 2021 Mar;21(3):1-1Read more>>
- Ying He. et al. Bioinformatics Profiling and Experimental Validation of 4 Differentially-Expressed LIM Genes in the Course of Colorectal-Adenoma-Carcinoma. MED SCI MONITOR. 2022; 28: e937081-1e937081-17Read more>>
- Wang Yan. et al. Qihuang Granule protects the retinal pigment epithelium from oxidative stress via regulation of the alternative complement pathway. BMC Complementary Medicine and Therapies. 2023 Dec;23(1):1-12Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Factor H Polyclonal Antibody, Unconjugated (bs-9525R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Factor H) Polyclonal Antibody, Unconjugated (bs-9525R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.