bs-9278R [Primary Antibody]
IRF3 (Ser386) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IRF3 Ser386

Modification Site: Ser386

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3661

Swiss Prot: Q14653

Source: KLH conjugated synthetic phosphopeptide derived from human IRF3 around the phosphorylation site of Ser386

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:50-200)
Flow-Cyt(Flow-Cyt=1ug/test)

Predicted Molecular Weight: 47


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zheng, Jiangang. et al. Curcumol inhibits encephalomyocarditis virus by promoting IFN- secretion. Bmc Vet Res. 2021 Dec;17(1):1-9Read more>>
  • Chen Fangzhao. et al. TRAF3 Positively Regulates Host Innate Immune Resistance to Influenza A Virus Infection. FRONT CELL INFECT MI. 2022 Apr;0:481Read more>>
  • Wang Shang. et al. Early activation of Toll-like receptor-3 reduces the pathological progression of Alzheimers disease in APP/PS1 mouse. ALZHEIMERS RES THER. 2023 Dec;15(1):1-17Read more>>
  • ingchen Zhang. et al. Precise RNA Editing: Cascade Self-Uncloaking Dual-Prodrug Nanoassemblies Based on CRISPR/Cas13a for Pleiotropic Immunotherapy of PD-L1-Resistant Colorectal Cancer. ADV FUNCT MATER. 2023 Sep;:230563Read more>>
  • Yatao Bao. et al. sNASP Mutation Aggravates to the TLR4-Mediated Inflammation in SLE by TAK1 Pathway. J IMMUNOL RES. 2023;2023:48777Read more>>
  • Hong-Fei Wang. et al. Jujuboside B alleviates acetaminophen-induced hepatotoxicity in mice by regulating Nrf2-STING signaling pathway. ECOTOX ENVIRON SAFE. 2024 Jan;269:11581Read more>>
  • Dadi Peng. et al. PPM1G regulates hepatic ischemia/reperfusion injury through STING-mediated inflammatory pathways in macrophages. IMMUN INFLAMM DIS. 2024 Feb;12(2):e1189Read more>>
  • Siyuan Luo. et al. Outer Membrane Vesicle-Wrapped Manganese Nanoreactor for Augmenting Cancer Metalloimmunotherapy through Hypoxia Attenuation and Immune Stimulation. ACTA BIOMATER. 2024 MayRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRF3 (Ser386) Polyclonal Antibody, Unconjugated (bs-9278R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRF3 (Ser386) Polyclonal Antibody, Unconjugated (bs-9278R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRF3 (Ser386) Polyclonal Antibody, Unconjugated (bs-9278R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRF3 (Ser386) Polyclonal Antibody, Unconjugated (bs-9278R) at 1:400 overnight at 4°C, DAB staining.