VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Mouse heart tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RNF169 Polyclonal Antibody, Unconjugated (bs-9259R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse heart tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RNF169 Polyclonal Antibody, Unconjugated (bs-9259R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAPI staining
Paraformaldehyde-fixed, paraffin embedded human cervical cancer tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RNF169 Polyclonal Antibody, Unconjugated (bs-9259R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse Cerebellum lysates probed with RNF169 Polyclonal Antibody, Unconjugated (bs-9259R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates probed with RNF169 Polyclonal Antibody, Unconjugated (bs-9259R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.