bs-9141R [Primary Antibody]
ZNRF3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ZNRF3

Immunogen Range: 101-200/936


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 84133

Swiss Prot: Q9ULT6

Source: KLH conjugated synthetic peptide derived from human ZNRF3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:50-200)

Predicted Molecular Weight: 101


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sarina Harshuk-Shabso. et al. Fgf and Wnt signaling interaction in the mesenchymal niche regulates the murine hair cycle clock. Nat Commun. 2020 Oct;11(1):1-14Read more>>
  • Federico Pecori. et al. Mucin-type O-glycosylation controls pluripotency in mouse embryonic stem cells via Wnt receptor endocytosis. J Cell Sci. 2020 Oct;133(20)Read more>>
  • Minghui Liu. et al. Comprehensive analysis of zinc and ring finger 3 in prognostic value and pan-cancer immunity. FASEB J. 2024 Mar;38(5):e23523Read more>>
VALIDATION IMAGES

Lane 1: Human HepG2 cell Lysates; Lane 2: Human A549 cell Lysates; Lane 3: Human U-2 OS cell Lysates. Probed with ZNRF3 polyclonal Antibody, unconjugated (bs-9141R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with ZNRF3 polyclonal antibody, unconjugated (bs-9141R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.