VALIDATION IMAGES
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-GPS1/CSN1 Polyclonal Antibody, Unconjugated (bs-9126R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti GPS1/CSN1 Polyclonal Antibody, Unconjugated (bs-9126R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 54kD. Observed band size: 54kD.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CSN1 Polyclonal Antibody, Unconjugated (bs-9126R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CSN1 Polyclonal Antibody, Unconjugated (bs-9126R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CSN1 Polyclonal Antibody, Unconjugated (bs-9126R) at 1:200 overnight at 4°C, DAB staining.