bs-8997R [Primary Antibody]
Annexin VI Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Annexin VI

Specificity: May have slight cross reactivity with ras-related protein Rap-2a due to 6 amino acids sequence similarity.

Immunogen Range: 601-673/673


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 309

Source: KLH conjugated synthetic peptide derived from human Annexin VI

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The Annexin family of calcium binding proteins is composed of at least ten mammalian genes. It is characterised by a conserved core domain which binds to phospholipids in a Ca2+ dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. When overexpressed in A431 cells, Annexin VI causes a partial reversal of the transformed phenotype.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse uterus lysates probed with Annexin VI Polyclonal Antibody, unconjugated (bs-8997R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Mouse spleen lysates probed with Rabbit Anti-Annexin VI Polyclonal Antibody, Unconjugated (bs-8997R) at 1:300 in 4˚C overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.


Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Liver tissue lysates probed with Annexin VI Polyclonal Antibody, Unconjugated (bs-8997R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.