bs-8929R-A350 [Conjugated Primary Antibody]
Cyclin E1 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cyclin E1

Immunogen Range: 251-350/410


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 898

Swiss Prot: P24864

Source: KLH conjugated synthetic peptide derived from human Cyclin E

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Conjugation: ALEXA FLUOR® 350

Excitation/ Emission: 346nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Chengke, and Zhenxin Wang. "Studying the relationship between cell cycle and Alzheimer’s disease by gold nanoparticle probes." Analytical Biochemistry (2015).Read more>>
  • Ren et al. Potential biomarkers of DNA replication stress in cancer. (2017) Oncotarget. 8:36996-37008Read more>>
  • Lv et al. Synthesis, biological evaluation and mechanism studies of deoxytylophorinine and its derivatives as potential anticancer agents. (2012) PLoS.On. 7:e30342Read more>>
  • Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017).Read more>>
VALIDATION IMAGES