bs-8878R [Primary Antibody]
LC3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LC3

Immunogen Range: 31-121/121


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 84557, 81631

Swiss Prot: Q9H492, Q9GZQ8

Source: KLH conjugated synthetic peptide derived from human LC3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Yunbo, et al. "β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice." Brain research 1552 (2014): 41-54.Read more>>
  • Li, Min, et al. "Metformin ameliorates hepatic steatosis and improves the induction of autophagy in HFD‑induced obese mice." Molecular Medicine Reports.Read more>>
  • Lu et al. Autophagy activator promotes neuronal differentiation of adult adipose-derived stromal cells. (2013) Neural.Regen.Res. 8:882-9Read more>>
  • Chen J et al. Tension induces intervertebral disc degeneration via endoplasmic reticulum stress-mediated autophagy. Biosci Rep. 2019 Jul 8. pii: BSR20190578.Read more>>
  • Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639. Read more>>
  • Jiaqi Lu. et al. Andrographolide emeliorates maltol aluminium-induced neurotoxicity via regulating p62-mediated Keap1-Nrf2 pathways in PC12 cells. Pharm Biol. 2021;59(1):232-241Read more>>
  • Jian Wang. et al. Lactobacillus salivarius ameliorated Mycoplasma gallisepticum-induced inflammatory injury and secondary Escherichia coli infection in chickens: Involvement of intestinal microbiota. Vet Immunol Immunop. 2021 Mar;233:110192Read more>>
  • Yukun Fang. et al. Activation of the ROS/HO-1/NQO1 signaling pathway contributes to the copper-induced oxidative stress and autophagy in duck renal tubular epithelial cells. Sci Total Environ. 2021 Feb;757:143753Read more>>
  • Maowen Chen. et al. Osteogenesis regulation of mesenchymal stem cells via autophagy induced by silicaCtitanium composite surfaces with different mechanical moduli. 2020 Sep 03Read more>>
  • Shaofeng Wu. et al. Protective effects of curcumin on ATO-induced nephrotoxicity in ducks in relation to suppressed autophagy, apoptosis and dyslipidemia by regulating oxidative stress. Ecotox Environ Safe. 2021 Aug;219:11235Read more>>
  • Xiaorui Fan. et al. Effect of Cryptorchidism on the Histomorphometry, Proliferation, Apoptosis, and Autophagy in Boar Testes. Animals-Basel. 2021 May;11(5):1379Read more>>
  • Xi Huaming. et al. Changes in histology, protein expression, and autophagy in dairy goat testes during non-breeding season. Biol Reprod. 2021 AugRead more>>
  • Shengsen Yang. et al. Effect and mechanism of action of SLP?2 on the apoptosis and autophagy of gastric cancer cells. Oncol Lett. 2021 Oct;22(4):1-8Read more>>
  • Fa-Xiu Chen. et al. Substance P prevents doxorubicin?induced cardiomyocyte injury by regulating apoptosis and autophagy: <em>In?vitro</em> and <em>in?vivo</em> evidence. Mol Med Rep. 2022 Feb;25(2):1-9Read more>>
  • Lang Xiu-Yuan. et al. Coeloglossum viride Var. Bracteatum Extract Attenuates MPTP-Induced Neurotoxicity in vivo by Restoring BDNF-TrkB and FGF2-Akt Signaling Axis and Inhibiting RIP1-Driven Inflammation. FRONT PHARMACOL. 2022 Apr;0:1571Read more>>
  • Yu-Shen Luo. et al. Acrylonitrile exposure triggers ovarian inflammation and decreases oocyte quality probably via mitochondrial dysfunction induced apoptosis in mice. CHEM-BIOL INTERACT. 2022 Jun;360:109934Read more>>
  • Jian Chen. et al. Astragalus polysaccharide alleviates transport stress-induced heart injury in newly hatched chicks via ERS-UPR-Autophagy dependent pathway. POULTRY SCIENCE. 2022 Jun;:10203Read more>>
  • Guo-Jian Jiang. et al. Ultraviolet B irradiation induces senescence of human corneal endothelial cells in vitro by DNA damage response and oxidative stress. J PHOTOCH PHOTOBIO B. 2022 Oct;235:112568Read more>>
  • Zhuo Ma. et al. Manganese-induced -synuclein overexpression promotes the accumulation of dysfunctional synaptic vesicles and hippocampal synaptotoxicity by suppressing Rab26-dependent autophagy in presynaptic neurons. SCI TOTAL ENVIRON. 2023 Feb;858:159753Read more>>
  • He Bai. et al. Inhibition of the BNIP3/NIX-dependent mitophagy aggravates copper-induced mitochondrial dysfunction in duck renal tubular epithelial cells. ENVIRON TOXICOL. 2022 NovRead more>>
  • Qian-Qian Hao. et al. Triptolide exposure induces oxidative stress and decreases oocyte quality in mouse. TOXICON. 2023 Jan;221:106964Read more>>
  • Yutong Wu. et al. Osteoclast-derived extracellular miR-106a-5p promotes osteogenic differentiation and facilitates bone defect healing. CELL SIGNAL. 2022 Dec;:110549Read more>>
  • Liu, Zhan. et al. Kaemperfol Protects Dopaminergic Neurons by Promoting mTOR-Mediated Autophagy in Parkinsons Disease Models. NEUROCHEM RES. 2022 Dec;:1-17Read more>>
  • Qidang Duan. et al. LOX-1 attenuates high glucose-induced autophagy via AMPK/HNF4 signaling in HLSECs. HELIYON. 2022 Dec;8:e12385Read more>>
  • Liu, Yanrong. et al. Cinnamaldehyde affects lipid droplets metabolism after adipogenic differentiation of C2C12 cells. MOL BIOL REP. 2022 Dec;:1-7Read more>>
  • Yi-Jing Yang. et al. <i>Lycium barbarum</i> Polysaccharides Regulating miR-181/Bcl-2 Decreased Autophagy of Retinal Pigment Epithelium with Oxidative Stress. OXID MED CELL LONGEV. 2023 Jan 05;2023:9554457Read more>>
  • Shimin Hao. et al. Evaluation of Biodegradable Alloy Fe30Mn0.6N in Rabbit Femur and Cartilage through Detecting Osteogenesis and Autophagy. BIOMED RES INT. 2023 Jan 18;2023:3626776Read more>>
  • Ying Huang. et al. Inhibitory Effect of Jinwujiangu Prescription on Peripheral Blood Osteoclasts in Patients with Rheumatoid Arthritis and the Relevant Molecular Mechanism. MEDIAT INFLAMM. 2023;2023:4814412Read more>>
  • Guo-Jian Jiang. et al. Carteolol triggers senescence via activation of -arrestinCERKCNOX4CROS pathway in human corneal endothelial cells in vitro. CHEM-BIOL INTERACT. 2023 Apr;:110511Read more>>
  • Linsong Yang. et al. Influence of Sodium New Houttuyfonate as a New EGFR-TK Inhibitor on the Apoptosis and Autophagy of MCF-7 Cells and Its Toxicity to Caenorhabditis elegans. PROCESSES. 2023 Jun;11(6):1652Read more>>
  • Lixiang Gu. et al. Mercuric chloride induced brain toxicity in mice: The protective effects of puerarin-loaded PLGA nanoparticles. J BIOCHEM MOL TOXIC. 2023 Jul;:e23425Read more>>
  • Yuko Naya. et al. Pathological study of proximal tubule mitochondria in diclofenac-induced acute kidney injury model mice. TISSUE CELL. 2023 Jul;:102188Read more>>
  • Huang Qun. et al. Bushenhuoluo Decoction improves polycystic ovary syndrome by regulating exosomal miR-30a-5p/ SOCS3/mTOR/NLRP3 signaling-mediated autophagy and pyroptosis. J OVARIAN RES. 2024 Dec;17(1):1-17Read more>>
  • Li Hongli. et al. Linc00707 regulates autophagy and promotes the progression of triple negative breast cancer by activation of PI3K/AKT/mTOR pathway. CELL DEATH DISCOV. 2024 Mar;10(1):1-14Read more>>
  • Wessam H. Elesawy. et al. Repurposing ezetimibe as a neuroprotective agent in a rotenone-induced Parkinsons disease model in rats: Role of AMPK/SIRT-1/PGC-1 signaling and autophagy. INT IMMUNOPHARMACOL. 2024 Sep;138:11264Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-LC3 Polyclonal Antibody, Unconjugated (bs-8878R) at 1:200 followed by conjugation to the secondary antibody (bs-0295G-HRP) and DAB staining.


Lane 1: mouse brain lysates probed with Rabbit Anti-LC3 Polyclonal Antibody, Unconjugated (bs-8878R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LC3 Polyclonal Antibody, Unconjugated (bs-8878R) at 1:200 overnight at 4°C, DAB staining.