bs-8877R-FITC [Conjugated Primary Antibody]
Somatostatin Polyclonal Antibody, FITC Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Somatostatin

Immunogen Range: 51-116/116


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6750

Swiss Prot: P61278

Source: KLH conjugated synthetic peptide derived from human Somatostatin

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Somatostatin inhibits the release of somatotropin.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)

Predicted Molecular Weight: 13


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Erika L Galer. et al. Cervical epidural electrical stimulation increases respiratory activity through somatostatin-expressing neurons in the dorsal cervical spine in rats. J NEUROSCI. 2022 DecRead more>>
VALIDATION IMAGES

Mouse Pancreas (C57BL/6 strain) fixed in 4% PFA for 2 hours at RT, then transferred to 30% sucrose overnight at 4°C before embedding in OCT mounting medium. Treated with TBS containing 5% goat serum and 0.3% Triton X-100, to block unspecific binding sites and permeabilize cell membranes, for 30min at RT. Antibody (bs-8877R-FITC) diluted at 1:100 in TBS containing 2% goat serum applied and incubated overnight at 4°C. Rinse sections in TBS 2x5min on a shaker, mount section with vectorshield (with DAPI), then cover them with coverslips and seal the edge of the coverslip on the slide with nail polish. This image was kindly submitted by an end-user from the National Childrens Research Centre, Our Ladys Childrens Hospital, Dublin, Ireland.