bs-8853R-BF594 [Conjugated Primary Antibody]
Smad2/Smad3 (Thr8) Polyclonal Antibody, AbBy Fluor® 594 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Smad2/Smad3 (Thr8)

Modification Site: Thr8

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4087

Swiss Prot: P84022

Source: KLH conjugated synthesised phosphopeptide derived from human Smad2/Smad3 around the phosphorylation site of Thr8

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.

Conjugation: AbBy Fluor® 594

Excitation/ Emission: 590nm/617nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen XY et al. Pulsed Magnetic Field Stimuli Can Promote Chondrogenic Differentiation of Superparamagnetic Iron Oxide Nanoparticles-Labeled Mesenchymal Stem Cells in Rats.(2018) J Biomed Nanotechnol. 14(12):2135-2145. Read more>>
  • Yan L et al. Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity. Cell Biology and Toxicology. Read more>>
  • Liang H et al. RGFP966, a Histone deacetylase 3 inhibitor, promotes glioma stem cell differentiation by blocking TGF- signaling via SMAD7. Biochem Pharmacol. 2020 Oct;180:114118.Read more>>
  • Xiaolan You. et al. Fibroblastic galectin-1-fostered invasion and metastasis are mediated by TGF-1-induced epithelial-mesenchymal transition in gastric cancer. Aging-Us. 2021 Jul 31; 13(14): 18464C18481Read more>>
  • Liang Yang. et al. TGF3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical Smad and atypical MAPK signaling pathways. ELECTRON J BIOTECHN. 2023 Jan;61:33Read more>>
  • Yijun Liu. et al. The development of nasal polyps involves early middle meatus mucous remodeling via TGF-1 mediated PAI-1 reduction. BRAZ J OTORHINOLAR. 2023 FebRead more>>
  • Xiaocong Jiang. et al. YES-associated protein-regulated Smad7 worsen epithelial barrier injury of chronic sinusitis with nasal polyps. IMMUN INFLAMM DIS. 2023 Jun;11(6):e907Read more>>
  • Chunyan Wan. et al. SMAD2/3 Phosphorylation is correlated with matrix metalloproteinase 9 expression in human periapical lesions. ARCH ORAL BIOL. 2023 Nov;155:105796Read more>>
  • Zhao Jiamin. et al. Sea buckthorn oil regulates primary myoblasts proliferation and differentiation in vitro. IN VITRO CELL DEV-AN. 2023 Dec;:1-12Read more>>
  • Yake Wang. et al. The Upregulation of Leucine-Rich Repeat Containing 1 Expression Activates Hepatic Stellate Cells and Promotes Liver Fibrosis by Stabilizing Phosphorylated Smad2/3. INT J MOL SCI. 2024 Jan;25(5):2735Read more>>
  • Hangzhuo Li. et al. Resistance exercise upregulates Irisin expression and suppresses myocardial fibrosis following myocardial infarction via activating AMPK-Sirt1 and inactivating TGF1-Smad2/3. ACTA PHYSIOL. 2024 May;:e14163Read more>>
  • Yao Hang. et al. miR-27b-3p reduces muscle fibrosis during chronic skeletal muscle injury by targeting TGF-R1/Smad pathway. J ORTHOP SURG RES. 2024 Dec;19(1):1-14Read more>>
  • Junfeng He. et al. Effect of the TGF-/BMP Signaling Pathway on the Proliferation of Yak Pulmonary Artery Smooth Muscle Cells under Hypoxic Conditions. ANIMALS. 2024 Jan;14(14):2072Read more>>
  • Zheng Wang. et al. Estrogen Deficiency Exacerbates Traumatic Heterotopic Ossification in Mice. J INFLAMM RES. 2024 22Read more>>
VALIDATION IMAGES