Host: Rabbit
Target Protein: Smad2/Smad3 (Thr8)
Modification Site: Thr8
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4087
Swiss Prot: P84022
Source: KLH conjugated synthesised phosphopeptide derived from human Smad2/Smad3 around the phosphorylation site of Thr8
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)
IF()
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Pig
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
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- Yan L et al. Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity. Cell Biology and Toxicology. Read more>>
- Liang H et al. RGFP966, a Histone deacetylase 3 inhibitor, promotes glioma stem cell differentiation by blocking TGF- signaling via SMAD7. Biochem Pharmacol. 2020 Oct;180:114118.Read more>>
- Xiaolan You. et al. Fibroblastic galectin-1-fostered invasion and metastasis are mediated by TGF-1-induced epithelial-mesenchymal transition in gastric cancer. Aging-Us. 2021 Jul 31; 13(14): 18464C18481Read more>>
- Liang Yang. et al. TGF3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical Smad and atypical MAPK signaling pathways. ELECTRON J BIOTECHN. 2023 Jan;61:33Read more>>
- Yijun Liu. et al. The development of nasal polyps involves early middle meatus mucous remodeling via TGF-1 mediated PAI-1 reduction. BRAZ J OTORHINOLAR. 2023 FebRead more>>
- Xiaocong Jiang. et al. YES-associated protein-regulated Smad7 worsen epithelial barrier injury of chronic sinusitis with nasal polyps. IMMUN INFLAMM DIS. 2023 Jun;11(6):e907Read more>>
- Chunyan Wan. et al. SMAD2/3 Phosphorylation is correlated with matrix metalloproteinase 9 expression in human periapical lesions. ARCH ORAL BIOL. 2023 Nov;155:105796Read more>>
- Zhao Jiamin. et al. Sea buckthorn oil regulates primary myoblasts proliferation and differentiation in vitro. IN VITRO CELL DEV-AN. 2023 Dec;:1-12Read more>>
- Yake Wang. et al. The Upregulation of Leucine-Rich Repeat Containing 1 Expression Activates Hepatic Stellate Cells and Promotes Liver Fibrosis by Stabilizing Phosphorylated Smad2/3. INT J MOL SCI. 2024 Jan;25(5):2735Read more>>
- Hangzhuo Li. et al. Resistance exercise upregulates Irisin expression and suppresses myocardial fibrosis following myocardial infarction via activating AMPK-Sirt1 and inactivating TGF1-Smad2/3. ACTA PHYSIOL. 2024 May;:e14163Read more>>
- Yao Hang. et al. miR-27b-3p reduces muscle fibrosis during chronic skeletal muscle injury by targeting TGF-R1/Smad pathway. J ORTHOP SURG RES. 2024 Dec;19(1):1-14Read more>>
- Junfeng He. et al. Effect of the TGF-/BMP Signaling Pathway on the Proliferation of Yak Pulmonary Artery Smooth Muscle Cells under Hypoxic Conditions. ANIMALS. 2024 Jan;14(14):2072Read more>>
- Zheng Wang. et al. Estrogen Deficiency Exacerbates Traumatic Heterotopic Ossification in Mice. J INFLAMM RES. 2024 22Read more>>
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃ and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad2/Smad3 (Thr8) Polyclonal Antibody(bs-8853R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).