DATASHEET
Host:
Rabbit
Target Protein:
HMG4/HMGB3
Immunogen Range:
1-100/200
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
3149
Swiss Prot:
O15347
Source:
KLH conjugated synthetic peptide derived from human HMG4/HMGB3:1-100/200
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM, Mar 2008]
Function : Binds preferentially single-stranded DNA and unwinds double stranded DNA.
Similarity : Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMG4/HMGB3 Polyclonal Antibody, Unconjugated (bs-8809R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMG4/HMGB3 Polyclonal Antibody, Unconjugated (bs-8809R) at 1:200 overnight at 4°C, DAB staining.