DATASHEET
Host:
Rabbit
Target Protein:
Beta Actin (Fruit Fly, Loading Control)
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
60
Swiss Prot:
P10987
Source:
Recombinant Fruit Fly Actin.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.
VALIDATION IMAGES
Lane 1: Fruit Fly lysates; Lane 2: Fruit Fly lysates probed with Actin (Fruit Fly) Polyclonal Antibody, Unconjugated (bs-8776R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Fruit Fly lysates; Lane 2: Fruit Fly lysates probed with Actin (Fruit Fly) Polyclonal Antibody, Unconjugated (bs-8776R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.