bs-8774R [Primary Antibody]
Histone H3 (CAEEL, Loading Control) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Histone H3 (CAEEL, Loading Control)

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 180074

Swiss Prot: P08898

Source: Recombinant Caenorhabditis elegans Histone H3.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 15


Cross Reactive Species: Caenorhabditis Elegans

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Caenorhabditis Elegans tissue lysates probed with Histone H3 (CAEEL) Polyclonal Antibody, Unconjugated (bs-8774R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.