bs-8707R-FITC

DATASHEET

Host: Rabbit

Target Protein: IRS1

Modification Site: Tyr632

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3667

Swiss Prot: P35568

Source: KLH conjugated Synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Tyr632

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)

Predicted Molecular Weight: 132

Cross Reactive Species: Human
Mouse
Rat
Bovine
Dog
Pig
Sheep
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.